Journal: Journal of Integrative Neuroscience

Article Title: FKBP51 is Involved in Epileptic Seizure by Regulating PSD95 in a PTZ-Induced Epileptic Mouse Model

doi: 10.31083/jin25710

Figure Lengend Snippet: Fig. 1. Expression of FKBP51 and SYP in PTZ and KA epileptic mouse models. (A,B) Expression of FKBP51 in PTZ and KA epileptic mouse models. (C–F) Expression of “total SYP and PSD95” and “SYP and PSD95 after extraction of synaptosomes” in the

Article Snippet: FKBP51 rabbit antibodies (1:1000, 14155-1-AP, Proteintech Group, Inc, Wuhan, China), PSD95 rabbit antibodies (1:5000, 20665-1-AP, Proteintech Group, Inc, Wuhan, China), SYP rabbit antibodies (1:40,000, Cat No. 17785-1-AP, Proteintech Group, Inc., Wuhan, China), GAPDH rabbit antibodies (1:10,000, 10494-1-AP, Proteintech Group, Inc, Wuhan, China), and β-tubulin mouse antibodies (1:50,000, 66240-1-Ig, Proteintech Group, Inc, Wuhan, China) were added and incubated overnight on a shaking table at 4 °C.

Techniques: Expressing, Extraction

Journal: Journal of Integrative Neuroscience

Article Title: FKBP51 is Involved in Epileptic Seizure by Regulating PSD95 in a PTZ-Induced Epileptic Mouse Model

doi: 10.31083/jin25710

Figure Lengend Snippet: Fig. 2. Localization of FKBP51 in epileptic brain tissue detected by immunofluorescence FKBP51 was expressed in the CA1 in the hippocampus of epileptic mice. White arrows indicate co-expression with GFAP and NeuN (n = 5). The scale bar represents 50

Article Snippet: FKBP51 rabbit antibodies (1:1000, 14155-1-AP, Proteintech Group, Inc, Wuhan, China), PSD95 rabbit antibodies (1:5000, 20665-1-AP, Proteintech Group, Inc, Wuhan, China), SYP rabbit antibodies (1:40,000, Cat No. 17785-1-AP, Proteintech Group, Inc., Wuhan, China), GAPDH rabbit antibodies (1:10,000, 10494-1-AP, Proteintech Group, Inc, Wuhan, China), and β-tubulin mouse antibodies (1:50,000, 66240-1-Ig, Proteintech Group, Inc, Wuhan, China) were added and incubated overnight on a shaking table at 4 °C.

Techniques: Immunofluorescence, Expressing

Journal: Journal of Integrative Neuroscience

Article Title: FKBP51 is Involved in Epileptic Seizure by Regulating PSD95 in a PTZ-Induced Epileptic Mouse Model

doi: 10.31083/jin25710

Figure Lengend Snippet: Fig. 3. Injection site and the selection of AAV-FKBP51-shRNA sequences. (A) According to the mouse atlas, the injection site is bilat-

Article Snippet: FKBP51 rabbit antibodies (1:1000, 14155-1-AP, Proteintech Group, Inc, Wuhan, China), PSD95 rabbit antibodies (1:5000, 20665-1-AP, Proteintech Group, Inc, Wuhan, China), SYP rabbit antibodies (1:40,000, Cat No. 17785-1-AP, Proteintech Group, Inc., Wuhan, China), GAPDH rabbit antibodies (1:10,000, 10494-1-AP, Proteintech Group, Inc, Wuhan, China), and β-tubulin mouse antibodies (1:50,000, 66240-1-Ig, Proteintech Group, Inc, Wuhan, China) were added and incubated overnight on a shaking table at 4 °C.

Techniques: Injection, Selection, shRNA

Journal: Journal of Integrative Neuroscience

Article Title: FKBP51 is Involved in Epileptic Seizure by Regulating PSD95 in a PTZ-Induced Epileptic Mouse Model

doi: 10.31083/jin25710

Figure Lengend Snippet: Fig. 4. Effects of FKBP51 on seizure grade and seizure latency in mice. (A,B) The seizure grades and latent period in the PTZ+AAV-

Article Snippet: FKBP51 rabbit antibodies (1:1000, 14155-1-AP, Proteintech Group, Inc, Wuhan, China), PSD95 rabbit antibodies (1:5000, 20665-1-AP, Proteintech Group, Inc, Wuhan, China), SYP rabbit antibodies (1:40,000, Cat No. 17785-1-AP, Proteintech Group, Inc., Wuhan, China), GAPDH rabbit antibodies (1:10,000, 10494-1-AP, Proteintech Group, Inc, Wuhan, China), and β-tubulin mouse antibodies (1:50,000, 66240-1-Ig, Proteintech Group, Inc, Wuhan, China) were added and incubated overnight on a shaking table at 4 °C.

Techniques:

Journal: Journal of Integrative Neuroscience

Article Title: FKBP51 is Involved in Epileptic Seizure by Regulating PSD95 in a PTZ-Induced Epileptic Mouse Model

doi: 10.31083/jin25710

Figure Lengend Snippet: Fig. 5. Effect of endogenous FKBP51 inhibition on apical dendritic spines in CA1 of the hippocampus. (A) The distribution of apical dendritic spines in the hippocampal CA1 and local enlarged images of the Con group, Model group, PTZ+AAV-GFP group, and

Article Snippet: FKBP51 rabbit antibodies (1:1000, 14155-1-AP, Proteintech Group, Inc, Wuhan, China), PSD95 rabbit antibodies (1:5000, 20665-1-AP, Proteintech Group, Inc, Wuhan, China), SYP rabbit antibodies (1:40,000, Cat No. 17785-1-AP, Proteintech Group, Inc., Wuhan, China), GAPDH rabbit antibodies (1:10,000, 10494-1-AP, Proteintech Group, Inc, Wuhan, China), and β-tubulin mouse antibodies (1:50,000, 66240-1-Ig, Proteintech Group, Inc, Wuhan, China) were added and incubated overnight on a shaking table at 4 °C.

Techniques: Inhibition

Journal: Journal of Integrative Neuroscience

Article Title: FKBP51 is Involved in Epileptic Seizure by Regulating PSD95 in a PTZ-Induced Epileptic Mouse Model

doi: 10.31083/jin25710

Figure Lengend Snippet: Fig. 6. Effect of endogenous FKBP51 inhibition on synaptic ultrastructure. (A) Clear synaptic structure (selected from Con group),

Article Snippet: FKBP51 rabbit antibodies (1:1000, 14155-1-AP, Proteintech Group, Inc, Wuhan, China), PSD95 rabbit antibodies (1:5000, 20665-1-AP, Proteintech Group, Inc, Wuhan, China), SYP rabbit antibodies (1:40,000, Cat No. 17785-1-AP, Proteintech Group, Inc., Wuhan, China), GAPDH rabbit antibodies (1:10,000, 10494-1-AP, Proteintech Group, Inc, Wuhan, China), and β-tubulin mouse antibodies (1:50,000, 66240-1-Ig, Proteintech Group, Inc, Wuhan, China) were added and incubated overnight on a shaking table at 4 °C.

Techniques: Inhibition

Journal: Journal of Integrative Neuroscience

Article Title: FKBP51 is Involved in Epileptic Seizure by Regulating PSD95 in a PTZ-Induced Epileptic Mouse Model

doi: 10.31083/jin25710

Figure Lengend Snippet: Fig. 7. Expression of PSD95 and SYP after decreasing the expression of FKBP51. (A–D) “Total PSD95 and SYP” and “PSD95

Article Snippet: FKBP51 rabbit antibodies (1:1000, 14155-1-AP, Proteintech Group, Inc, Wuhan, China), PSD95 rabbit antibodies (1:5000, 20665-1-AP, Proteintech Group, Inc, Wuhan, China), SYP rabbit antibodies (1:40,000, Cat No. 17785-1-AP, Proteintech Group, Inc., Wuhan, China), GAPDH rabbit antibodies (1:10,000, 10494-1-AP, Proteintech Group, Inc, Wuhan, China), and β-tubulin mouse antibodies (1:50,000, 66240-1-Ig, Proteintech Group, Inc, Wuhan, China) were added and incubated overnight on a shaking table at 4 °C.

Techniques: Expressing